Granulocyte colony-stimulating factor (GCSF) fused with Fc Domain produced from E. coli is less effective than Polyethylene Glycol-conjugated GCSF
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چکیده
منابع مشابه
Efficient Process Development of Recombinant Human Granulocyte Colony-Stimulating Factor (rh-GCSF) Production in Escherichia coli
Background: The protein hormone granulocyte colony-stimulating factor (GCSF) stimulates the production of white blood cells and plays an important role in medical treatment of cancer patients. Methods: An efficient process was developed for heterologous expression of human GCSF in E. coli BL21 (DE3). The feeding rate was adjusted to achieve the maximum attainable specific growth rate under crit...
متن کاملExpression of granulocyte colony stimulating factor (GCSF) in Hansenula polymorpha
BACKGROUND AND OBJECTIVES During past decades Hansenula polymorpha has attracted global attention for the expression of recombinant proteins due to its high growth rate, minimal nutritional porequirements and use of methanol as a low cost inducer. MATERIALS AND METHODS The corresponding nucleotide sequences for the expression of heterologous genes in Hansenula poylmorpha were extracted and as...
متن کاملEfficient Process Development of Recombinant Human Granulocyte Colony-Stimulating Factor (rh-GCSF) Production in Escherichia coli
BACKGROUND The protein hormone granulocyte colony-stimulating factor (GCSF) stimulates the production of white blood cells and plays an important role in medical treatment of cancer patients. METHODS An efficient process was developed for heterologous expression of the human GCSF in E. coli BL21 (DE3). The feeding rate was adjusted to achieve the maximum attainable specific growth rate under ...
متن کاملexpression of granulocyte colony stimulating factor (gcsf) in hansenula polymorpha
background and objectives: during past decades hansenula polymorpha has attracted global attention for the expression of recombinant proteins due to its high growth rate, minimal nutritional porequirements and use of methanol as a low cost inducer. materials and methods: the corresponding nucleotide sequences for the expression of heterologous genes in hansenula poylmorpha were extracted and as...
متن کاملefficient process development of recombinant human granulocyte colony-stimulating factor (rh-gcsf) production in escherichia coli
background: the protein hormone granulocyte colony-stimulating factor (gcsf) stimulates the production of white blood cells and plays an important role in medical treatment of cancer patients. methods: an efficient process was developed for heterologous expression of human gcsf in e. coli bl21 (de3). the feeding rate was adjusted to achieve the maximum attainable specific growth rate under crit...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2017
ISSN: 2045-2322
DOI: 10.1038/s41598-017-06726-7